IHC - BOB1

IHC – BOB1 Test

The IHC BOB1 Test is an advanced immunohistochemistry (IHC) diagnostic test used to detect BOB1 protein expression in tissue samples. BOB1 (B-cell Oct-binding protein 1) is an important B-cell-specific transcription coactivator that plays a critical role in normal B-cell development and function.

This test is primarily used in hematopathology and lymphoma diagnosis to identify B-cell lineage in lymphoid tumors, helping pathologists distinguish B-cell lymphomas from other lymphoid or non-lymphoid malignancies.

BOB1 is highly valuable in diagnosing classical Hodgkin lymphoma, diffuse large B-cell lymphoma (DLBCL), follicular lymphoma, mantle cell lymphoma, Burkitt lymphoma, and other B-cell neoplasms. It is often used as part of a broader immunohistochemistry panel to confirm tumor classification and support accurate diagnosis.

The test is usually performed on FFPE (Formalin-Fixed Paraffin-Embedded) tissue biopsy samples using advanced antibody staining techniques. Expert pathologists examine stained tissue microscopically to assess BOB1 protein expression patterns and determine whether the tumor has B-cell origin.

BOB1 testing is particularly useful when routine histopathology findings are inconclusive or when detailed lymphoma subclassification is required for treatment planning.

Accurate identification of B-cell lineage helps oncologists determine prognosis and select the most effective targeted therapies.

Benefits of the Test

• Detects B-cell lineage accurately
• Helps classify lymphoid tumors
• Supports lymphoma subtype diagnosis
• Improves pathology diagnostic precision
• Assists treatment planning
• Supports personalized hematology care

Why Doctors Recommend This Test

Doctors commonly recommend the BOB1 IHC Test when:

• B-cell lymphoma is suspected
• Lymphoma subtype classification is needed
• Routine biopsy findings are inconclusive
• Hodgkin lymphoma evaluation is required
• Differential diagnosis of lymphoid tumors is necessary
• Advanced hematopathology confirmation is needed
• Treatment planning depends on exact diagnosis
• Tumor lineage clarification is essential

This test improves diagnostic confidence for accurate hematologic cancer management.

Preparation Before the Test

Preparation usually includes:

• Submission of FFPE tissue block or unstained slides
• Previous pathology reports
• Relevant imaging and clinical records
• Proper tissue labeling and packaging
• Laboratory authorization documentation

No fasting or direct patient preparation is required.

Normal Reporting Time

The normal reporting time for the BOB1 IHC Test is usually 4–7 working days, depending on staining complexity and expert interpretation requirements.

Who Should Take This Test?

This test is recommended for:

• Patients with suspected lymphoma
• Individuals requiring B-cell lineage confirmation
• Patients undergoing advanced hematopathology evaluation
• Individuals with unclear lymphoid tumor diagnosis
• Oncology patients under specialist care
• Patients requiring expert lymphoma classification

At Focus Diagnostics Hyderabad, advanced immunohistochemistry technology and expert hematopathology specialists ensure highly accurate BOB1 IHC reports for reliable diagnosis and personalized cancer treatment planning.